View online or download bd accuri c6 instrument manual. Bd biosciences bd accuri c6 plus community, manuals and. To recreate bd accuri c6 software plots in flowjo with the same axes scaling as the original bd accuri c6 software file, note the channel range used to view each parameter figure 1. The flow cytometry unit at university of helsinki integrates three flow cytometry facilities located at the meilahti and viikki campuses. Data was generated on either the bd accuri c6 plus or the bd accuri c6 as indicated in figure legends. Turn on the computer and log in with your ucf info.
Any one have good or bad experiences with the new bd. Gate names are assigned sequentially by type r1, r2 r3, p1, p2, p3, m1, m2 etc. The flow rate, relative to sample concentration, should minimize doublets and larger. Comparison and evaluation of seven different bench. Accuri c6 maintenance cleaning and decontamination. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. If you have questions or require further clarification, see the bd accuri c6 flow cytometer instrument manual or contact accuri technical support. Accuri c6 is a digital pc based bench top analyser, 2 laser system. Technical bulletin a guide to absolute counting using the bd accuri c6 flow cytometer bd biosciences august 2011 the ideal cell concentration range for accurate counting varies among cell types, based on considerations such as size, shape, and tendency to clump. The accuri cflow or cflow plus software cflow allows you to control the c6 flow cytometer system to acquire data, generate statistics, and analyze results. Accuri 6 flow cytometer, monitor, keyboard, mouse, hard drive, waste bottle, sheath bottle, decontamination bottle, cleaning bottle, all cflow software disks, and manual.
Bd accuri c6 flow cytometer instrument manual bd biosciences. The system is equipped with blue 488 nm and red 640nm lasers, four variable fluorescence detectors and. The accuri cflow or cflow plus software cflow allows you to control the c6. Bd accuri c6 software user guide manual 782009501 rev0 iii f. It is the responsibility of the userbuyer to install and maintain antivirus software on the computer workstation. To fill the cleaning solution bottle green line, add 3 ml of 1030% citric acid and fill with di. Press the power button on the bd accuri c6 instrument face panel. Easytouse software with automated qc and application templates bd accuri c6 plus software features an intuitive user interface. It uses a bunch of new technology that bd has built to address usability software and reliability hardware. What you see in the pictures is what you will be receiving and please feel free. Bd accuri c6 flow cytometer instrument manual bd accuri c6 software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. Software options and instrument controls are clearly visible from the softwares tabbed interface. Buy from accuri, or make your own citric acid solution. Many bd accuri c6 customers can begin collecting and analyzing data with the help of a quick start guide.
This portable and compact system holds the analytical power of a cytometer twice its size. Nov 09, 2017 optimize your flow cytometry best practices for sample preparation, staining and analysis duration. Start the c6 flow cytometer, open bd accuri c6 software and wait for the green traffic light message. Does anyone have any recommendations for free software. Waste and sheath fluid bottles were checked during this time, and emptied or refilled as required. Hi as far as i know, there is nothing that bd can do to overcome this issue on accuri c6 itself by bd claims that they have used larger valves and thicker tubing system in accuri c6 plus and facsvia ivd version to cope with this problem. Msds linked at the right cleaning solution, kr225 citric acid. Facsdiva v6 software overview course bd biosciences accuri c6 software user guide.
Accuri c6 maintenance cleaning and decontamination alternatives. Bd accuri c6 with plate handler april 2016 this protocol contains instructions for setting up an accuri c6 with plate handler. Bd accuri c6 software controls the bd accuri c6 flow cytometer system in order. Accuri c6 configuration university of rochester medical. The c6 contains two collinear lasers and is arranged in such a way that four detectors all simultaneously measure light scatter.
Bd accuri c6 flow cytometer instrument manual 782001801 rev2 1 the bd accuri c6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Bd accuri c6 flow cytometer instrument manual 7820018 rev4 1 1 introduction the bd accuri c6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Acquired in 2015, the bd accuri system is a highquality, simple to use, flow cytometer capable of measurements of eukaryotic and prokaryotic cells. Fcs express will import all of the experiment information from the bd accuri c6 software files including all plots on the collect and analyze tab, the native high resolution 24 bit fcs data, all gates, gating hierarchies, markers, quadrants, plot formatting and scaling settings.
Bd accuri cytometers use the cflow software, which produces fcs 3. A new workspace can be used to create an analysis template and to collect a new dataset. Always save bd accuri c6 data as a bd csampler software file. The bd accuri c6 plus personal flow cytometer is the newest generation of the bd accuri platform. Bd accuri c6 flow cytometer instrument manual 7820018 rev2 3 refer to the bd accuri c6 safety and limitations manual pn 7820091 for complete safety information. We offer stateofart analyzing and sorting flow cytometers for use to universityassociated and external researchers. Bd accuri c6 plus software features an intuitive user interface. Install, update, and run antivirus protection software on a regular basis. The bd accuri c6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. Bd accuri c6 flow cytometer quick set up guide umces. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour.
Flow cytometer system to acquire data, generate statistics, and analyze results. Documentation bd accuri software user guide bd accuri user manual bd accuri user instructions installed in 2015. All reagents and kits are compatible with both the bd accuri c6 plus and bd accuri c6 flow cytometer systems. The bd accuri c6 flow cytometer was switched on and allowed to warm up for 15 minutes before analyses were begun. Using the bd accuri c6 cytometer for rapid and accurate. Bd csampler software user guide iv 782009901 rev2 table of figures figure 11. Figure 1a illustrates the overall standard cure conformity among the seven b. Operate the instrument as described in this instruction manual.
Bd accuri c6 flow cytometry system available for sale. A bd csampler software file is a comprehensive and often large data file that contains instrument settings, fcs files, and plot layouts. Designed from the ground up to be compact and easy to use, the bd accuri c6 flow cytometer fits in any lab and is readily accessible. Bd accuri c6 flow cytometer quick set up guide please follow these directions for setting up your bd accuri c6 flow cytometer. We have 1 bd accuri c6 manual available for free pdf download. It also includes common troubleshooting help and service information.
For details on saving a bd csampler software file, see section 4. Examples of axes ranges used in a bd accuri c6 software file. Accuri c6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to facscalibur. Manual adjustment for gain on the laser exciting the reporter molecule was not possible for instruments a and f.
Any one have good or bad experiences with the new bd accuri c6 plus. David kranzs lab click link for campus map is equipped with an accuri c6 4color flow cytometry analyzer. The bd accuri c6 software installer is on the cd that came with the c6. Feb 20, 2017 bd accuri c6 flow cytometry system available for sale. The c6 software status message box c6 is connected and ready. The bd accuri c6 software importer allows you to import your entire experiment from accuri c6 and c6 plus systems. When setting up the cytometer, note the details in the following sections to maximize the instruments performance. Tabbed views for collection, analysis, and statistics. Cell cycle analysis in bd accuri c6 bio technologies. Only operate the accuri c6 flow cytometer with an original ed version of software authorized by accuri. Accuri c6 software automatically displays the percentage of cells within the region. The bd facsvia flow cytometry system is an easytouse benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement, laborintensive manual procedures, fewer resources and higher demands for efficiency, so your laboratory runs smoothly and efficiently.
Bd accuri c6 software will display this notification based on whichever trigger is. Standard fluorochrome in use are identical to facs calibur. Analytical flow cytometry accuri c6 configuration please note that the accuri c6 is for use by training only. Cited in over 9,000 publications, the bd accuri c6 plus enables you to go beyond your current research potential. Hi all, we have a very gently used bd accuri c6 cytometer with csampler. Ive recently started doing flow cytometry bd accuri c6 in a core facility at my university, but my lab doesnt have any softtware to analyze the data. Take a leap in flow cytometry with the bd accuri c6 plus. Accuri c6 flow cytometry analyzer the satelite facility at 348 burrill hall dr. When we dont have any notion, thats pretty harsh to follow. Accuri cytometers cflow user guide usf biological sciences. The software for accuri c6 cflow plus is very userfriendly and efficient. Collect the collect tab is used to collect or import a sample, configure the run settings and set up plots and gates. The bd accuri c6 flow cytometer system includes bd accuri c6 software for controlling the instrument, generating statistics, and analyzing results. Search results for instrument setup and beads on bd biosciencesus.
Lin fsch, channels 0 to 5 x 107 lin ssch, channels 0 to 2x 107. Bd accuri c6 with single tube loader protocol this protocol contains instructions for setting up an accuri c6 with tube loader. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. The intuitive interface of the software guides the user through workflows. In addition, c6 has a flow metering system for automatic calculation of absolute cell counts. An assessment of software for flow cytometry analysis in banana plants. Gates cannot only be deleted from the workspace by deleting the plot on which it was drawn. Our neuroimmunology lab currently has a bd accuri c6 that has broken about every year and a half since its purchase about 5. For information on using bd accuri c6 software, see the bd accuri c6 software. Accuri c6 is an easy to use flow cytometer which can be connected to an autosampler 48 and 96 well, and 24 tube rack, we do not have one. Breakthrough with the bd accuri c6 plus cell analyzer.
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